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fgf10  (R&D Systems)


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    Structured Review

    R&D Systems fgf10
    Fgf10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+fgf10/Recombinant+Human+FGF-10+Protein/pm41997918-303-65-67
    Average 96 stars, based on 183 article reviews
    fgf10 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: Decreased lncRNA HNF4A-AS1 facilitates resistance to sorafenib-induced ferroptosis of hepatocellular carcinoma by reprogramming lipid metabolism.
    Article Snippet: .. 14, Issue 18 https://www.thno.org 7105 base medium, 1:50 N21-MAX Media Supplement (50X) (R&D systems, USA, AR008), 1:100 N-2 MAX Media Supplement (100X) (R&D systems, USA, AR009), 1.25mM N-acetyl-L-cysteine (MCE, USA, HY-B0215), 10mM nicotinamide (MCE, USA, HY-B0150), 10nM (Leu15) gastrin I (R&D systems, USA, 3006/1), 50 ng/ml recombinant human EGF (R&D systems, USA, 236-EG), 100 ng/ml recombinant human FGF10 (R&D systems, USA, 345-FG), 25 ng/ml recombinant human HGF (R&D systems, USA, 294-HGN-025/CF), 10 μM forskolin (Tocris, USA, 1099/10), 5 μM A8301 (Tocris, USA, 2939/50), 3nM dexamethasone (Sigma, USA, D4902-25MG) and 10μM Y27632 (MCE, USA, HY-10071). .. The expansion medium (EM) contains IM without dexamethasone and supplement with 500ng/ml recombinant human r-spondin1 (R&D systems, USA, 4645-RS-100/CF).

    Article Title: Ovarian Cancer Patient-Derived Organoids Used as a Model for Replicating Genetic Characteristics and Testing Drug Responsiveness: A Preliminary Study.
    Article Snippet: The basal medium was called AdDF+++ media and was composed of DMEM/ F12 with 1× Glutamax (Sigma), 10 mM HEPES (Sigma), and penicillin/streptomycin (Sigma). .. The organoid culture media consisting of AdDF+++ supplemented with 1× B27 supplement (Life Technologies, Paisley, UK), 1.25 mM N-acetyl-l-cysteine (Sigma-Aldrich, MO, USA), 50 ng/mL WNT3A (R&D Systems, MN, USA), 250 ng/mL R-spondin1 (R&D Systems), 100 ng/mL Noggin (R&D Systems), 5 mM nicotinamide (Sigma-Aldrich), 50 ng/mL recombinant human EGF (epidermal growth factor, R&D Systems), 100 ng/mL recombinant human FGF10 (R&D Systems), 10 μM forskolin (R&D Systems), 5 μM A8301 (Tocris Bioscience, Bristol, UK), 500 ng/mL hydrocortisone (Sigma-Aldrich), 37.5 ng/mL Heregulinβ-1 (R&D Systems), 100 nM β-Estradiol (Sigma-Aldrich), and 10 μM Y27632 (Merck Millipore). .. Cell viability was assessed using Cell Titer Glow 3D Reagent (Promega, WI, USA).

    Article Title: 14-3-3 binding motif phosphorylation disrupts Hdac4-organized condensates to stimulate cardiac reprogramming
    Article Snippet: 1 μg/mL puromycin (SIGP8833-25MG, Sigma) was added into the medium 3 days after infection to eliminate cells without infection. .. For spontaneous beating and calcium transient assessment experiments, induction medium was replaced every 2–3 days by mature medium containing StemPro-34 SF medium (Gibco, Thermo Fisher Scientific), GlutaMAX (10 μL/mL, Gibco, Thermo Fisher Scientific), ascorbic acid (50 μg/mL, Sigma), recombinant human VEGF165 (5 ng/mL, R&D Systems), recombinant human FGF10 (25 ng/mL, R&D Systems), and recombinant human FGF basic146 aa (10 ng/mL, R&D Systems) for another 2 weeks. ..

    Article Title: A serum-free culture medium for long-term expansion of human airway basal stem cells
    Article Snippet: The base medium was composed of a 1:1 mixture of Iscove’s Modified Dulbecco Medium (IMDM, Gibco, 12440061) and Ham’s F12 (Gibco, 11765054). .. The complete sfBSC medium was supplemented with the following components: 1X B27 supplement (Gibco, 17504044), 0.1% recombinant human serum albumin (rHSA, Sigma-Aldrich, A9731), 2 mM Glutamax (Gibco, 35050061), 50 μg/mL L-ascorbic acid (Sigma-Aldrich, A4544), 0.4 μM monothioglycerol (MTG, Sigma-Aldrich, M6145), 1 μg/mL hydrocortisone (STEMCELL Technologies, 07926), 1 ng/mL recombinant human EGF (Sigma-Aldrich, SRP3027), 0.4 ng/mL recombinant human FGF10 (R&D systems, 345-FG), 10 μM Y27632 (Rho-associated protein kinase (ROCK) inhibitor, Selleck, S1049), 1 μM DAPT (γ-secretase/Notch signaling pathway inhibitor, Sigma-Aldrich, D5942), 1 μM A83-01 (ALK5 inhibitor, Sigma-Aldrich, SML0788), and 1 μM DMH-1 (BMP signaling inhibitor, Sigma-Aldrich, D8946). ..

    Article Title: 14-3-3 binding motif phosphorylation disrupts Hdac4-organized condensates to stimulate cardiac reprogramming.
    Article Snippet: 1 mg/mL puromycin (SIGP8833-25MG, Sigma) was added into themedium 3 days after infection to eliminate cells without infection. .. For spontaneous beating and calcium transient assessment experiments, induction medium was replaced every 2–3 days by mature medium containing StemPro-34 SF medium (Gibco, Thermo Fisher Scientific), GlutaMAX (10 mL/mL, Gibco, Thermo Fisher Scientific), ascorbic acid (50 mg/mL, Sigma), recombinant human VEGF165 (5 ng/mL, R&D Systems), recombinant human FGF10 (25 ng/mL, R&D Systems), and recombinant human FGF basic146 aa (10 ng/mL, R&D Systems) for another 2 weeks.63 ..

    Article Title: Transient BRD4 degradation improves cardiac reprogramming by inhibiting macrophage/Oncostatin M induced JAK/STAT pathway
    Article Snippet: 1 μg/ml puromycin (SIGP8833-25MG, Sigma) was added into the medium 3 days after infection to eliminate cells without infection. .. For spontaneous beating and calcium transient assessment experiments, induction medium was replaced every 2-3 days by mature medium containing StemPro-34 SF medium (Gibco, Thermo Fisher Scientific), GlutaMAX (10 μl/ml, Gibco, Thermo Fisher Scientific), ascorbic acid (50 μg/ml, Sigma), recombinant human VEGF165 (5 ng/ml, R&D Systems), recombinant human FGF10 (25 ng/ml, R&D Systems), and recombinant human FGF basic146 aa (10 ng/ml, R&D Systems) for another 2 weeks as previously described . ..

    Article Title: Ovarian Cancer Patient-Derived Organoids Used as a Model for Replicating Genetic Characteristics and Testing Drug Responsiveness: A Preliminary Study
    Article Snippet: The basal medium was called AdDF+++ media and was composed of DMEM/F12 with 1× Glutamax (Sigma), 10 mM HEPES (Sigma), and penicillin/streptomycin (Sigma). .. The organoid culture media consisting of AdDF+++ supplemented with 1× B27 supplement (Life Technologies, Paisley, UK), 1.25 mM N-acetyl-l-cysteine (Sigma-Aldrich, MO, USA), 50 ng/mL WNT3A (R&D Systems, MN, USA), 250 ng/mL R-spondin1 (R&D Systems), 100 ng/mL Noggin (R&D Systems), 5 mM nicotinamide (Sigma-Aldrich), 50 ng/mL recombinant human EGF (epidermal growth factor, R&D Systems), 100 ng/mL recombinant human FGF10 (R&D Systems), 10 μM forskolin (R&D Systems), 5 μM A8301 (Tocris Bioscience, Bristol, UK), 500 ng/mL hydrocortisone (Sigma-Aldrich), 37.5 ng/mL Heregulinβ-1 (R&D Systems), 100 nM β-Estradiol (Sigma-Aldrich), and 10 μM Y27632 (Merck Millipore). .. Cell viability was assessed using Cell Titer Glow 3D Reagent (Promega, WI, USA).

    Article Title: Decreased lncRNA HNF4A-AS1 facilitates resistance to sorafenib-induced ferroptosis of hepatocellular carcinoma by reprogramming lipid metabolism
    Article Snippet: .. The initiation medium (IM) consists of base medium, 1:50 N21-MAX Media Supplement (50X) (R&D systems, USA, AR008), 1:100 N-2 MAX Media Supplement (100X) (R&D systems, USA, AR009), 1.25mM N-acetyl-L-cysteine (MCE, USA, HY-B0215), 10mM nicotinamide (MCE, USA, HY-B0150), 10nM (Leu15) gastrin I (R&D systems, USA, 3006/1), 50 ng/ml recombinant human EGF (R&D systems, USA, 236-EG), 100 ng/ml recombinant human FGF10 (R&D systems, USA, 345-FG), 25 ng/ml recombinant human HGF (R&D systems, USA, 294-HGN-025/CF), 10 μM forskolin (Tocris, USA, 1099/10), 5 μM A8301 (Tocris, USA, 2939/50), 3nM dexamethasone (Sigma, USA, D4902-25MG) and 10μM Y27632 (MCE, USA, HY-10071). .. The expansion medium (EM) contains IM without dexamethasone and supplement with 500ng/ml recombinant human r-spondin1 (R&D systems, USA, 4645-RS-100/CF).



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    Evaluation of the effects of specific components in sfBSC medium on the growth of HBECs. A Brightfield images showing the morphology of HBECs at P3 and P4 after the removal of B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. B Quantitative assessment of HBEC proliferation using the CCK-8 assay following the removal of individual components (B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF) from the sfBSC medium ( n = 3 per group). C Growth curves showing changes in cell numbers over time following the removal of rHSA, hydrocortisone or <t>FGF10</t> from the sfBSC medium ( n = 3 per group). D Quantification of colony formation from single-cell seeding follwoing the removal of rHSA, hydrocortisone, or FGF10 ( n = 3 per group). E Brightfield images showing the morphology of HBECs at P3 to P6 after the removal of A-83-01, DMH1, or DAPT from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. White boxes highlight regions containing differentiated cells. F cPD curves for HBECs at different passages following removal of A-83-01, DMH1, or DAPT in sfBSC medium. Statistical significance at last day was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test vs. complete medium; ***p < 0.001
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    Evaluation of the effects of specific components in sfBSC medium on the growth of HBECs. A Brightfield images showing the morphology of HBECs at P3 and P4 after the removal of B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. B Quantitative assessment of HBEC proliferation using the CCK-8 assay following the removal of individual components (B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF) from the sfBSC medium ( n = 3 per group). C Growth curves showing changes in cell numbers over time following the removal of rHSA, hydrocortisone or <t>FGF10</t> from the sfBSC medium ( n = 3 per group). D Quantification of colony formation from single-cell seeding follwoing the removal of rHSA, hydrocortisone, or FGF10 ( n = 3 per group). E Brightfield images showing the morphology of HBECs at P3 to P6 after the removal of A-83-01, DMH1, or DAPT from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. White boxes highlight regions containing differentiated cells. F cPD curves for HBECs at different passages following removal of A-83-01, DMH1, or DAPT in sfBSC medium. Statistical significance at last day was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test vs. complete medium; ***p < 0.001
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    Evaluation of the effects of specific components in sfBSC medium on the growth of HBECs. A Brightfield images showing the morphology of HBECs at P3 and P4 after the removal of B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. B Quantitative assessment of HBEC proliferation using the CCK-8 assay following the removal of individual components (B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF) from the sfBSC medium ( n = 3 per group). C Growth curves showing changes in cell numbers over time following the removal of rHSA, hydrocortisone or <t>FGF10</t> from the sfBSC medium ( n = 3 per group). D Quantification of colony formation from single-cell seeding follwoing the removal of rHSA, hydrocortisone, or FGF10 ( n = 3 per group). E Brightfield images showing the morphology of HBECs at P3 to P6 after the removal of A-83-01, DMH1, or DAPT from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. White boxes highlight regions containing differentiated cells. F cPD curves for HBECs at different passages following removal of A-83-01, DMH1, or DAPT in sfBSC medium. Statistical significance at last day was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test vs. complete medium; ***p < 0.001
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    Evaluation of the effects of specific components in sfBSC medium on the growth of HBECs. A Brightfield images showing the morphology of HBECs at P3 and P4 after the removal of B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. B Quantitative assessment of HBEC proliferation using the CCK-8 assay following the removal of individual components (B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF) from the sfBSC medium ( n = 3 per group). C Growth curves showing changes in cell numbers over time following the removal of rHSA, hydrocortisone or FGF10 from the sfBSC medium ( n = 3 per group). D Quantification of colony formation from single-cell seeding follwoing the removal of rHSA, hydrocortisone, or FGF10 ( n = 3 per group). E Brightfield images showing the morphology of HBECs at P3 to P6 after the removal of A-83-01, DMH1, or DAPT from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. White boxes highlight regions containing differentiated cells. F cPD curves for HBECs at different passages following removal of A-83-01, DMH1, or DAPT in sfBSC medium. Statistical significance at last day was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test vs. complete medium; ***p < 0.001

    Journal: Stem Cell Research & Therapy

    Article Title: A serum-free culture medium for long-term expansion of human airway basal stem cells

    doi: 10.1186/s13287-026-04910-z

    Figure Lengend Snippet: Evaluation of the effects of specific components in sfBSC medium on the growth of HBECs. A Brightfield images showing the morphology of HBECs at P3 and P4 after the removal of B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. B Quantitative assessment of HBEC proliferation using the CCK-8 assay following the removal of individual components (B27 supplement, GlutaMAX, L-ascorbic acid, Y27632, or EGF) from the sfBSC medium ( n = 3 per group). C Growth curves showing changes in cell numbers over time following the removal of rHSA, hydrocortisone or FGF10 from the sfBSC medium ( n = 3 per group). D Quantification of colony formation from single-cell seeding follwoing the removal of rHSA, hydrocortisone, or FGF10 ( n = 3 per group). E Brightfield images showing the morphology of HBECs at P3 to P6 after the removal of A-83-01, DMH1, or DAPT from the sfBSC medium. Complete sfBSC medium served as a control. Scale bar = 200 μm. White boxes highlight regions containing differentiated cells. F cPD curves for HBECs at different passages following removal of A-83-01, DMH1, or DAPT in sfBSC medium. Statistical significance at last day was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test vs. complete medium; ***p < 0.001

    Article Snippet: The complete sfBSC medium was supplemented with the following components: 1X B27 supplement (Gibco, 17504044), 0.1% recombinant human serum albumin (rHSA, Sigma-Aldrich, A9731), 2 mM Glutamax (Gibco, 35050061), 50 μg/mL L-ascorbic acid (Sigma-Aldrich, A4544), 0.4 μM monothioglycerol (MTG, Sigma-Aldrich, M6145), 1 μg/mL hydrocortisone (STEMCELL Technologies, 07926), 1 ng/mL recombinant human EGF (Sigma-Aldrich, SRP3027), 0.4 ng/mL recombinant human FGF10 (R&D systems, 345-FG), 10 μM Y27632 (Rho-associated protein kinase (ROCK) inhibitor, Selleck, S1049), 1 μM DAPT (γ-secretase/Notch signaling pathway inhibitor, Sigma-Aldrich, D5942), 1 μM A83-01 (ALK5 inhibitor, Sigma-Aldrich, SML0788), and 1 μM DMH-1 (BMP signaling inhibitor, Sigma-Aldrich, D8946).

    Techniques: Control, CCK-8 Assay, Single Cell

    Optimization of EGF and FGF10 concentrations for maintaining BSC morphology. A Brightfield images showing the morphology of HBECs cultured in media supplemented with different concentrations of EGF. Scale bar = 200 μm. White boxes demarcate cells exhibiting morphological alterations. B Brightfield images showing the morphology of HBECs cultured in media supplemented with different concentrations of FGF10. Scale bar = 200 μm. White boxes demarcate cells exhibiting morphological alterations. C Immunofluorescence staining of P63 and KRT5 in HBECs grown in medium supplemented with 40 ng/mL FGF10. Scale bar = 50 μm. D Immunofluorescence staining of NGFR in HBECs grown in media supplemented with 40 ng/mL FGF10. White borders demarcate regions with altered NGFR expression patterns. Scale bar = 50 μm

    Journal: Stem Cell Research & Therapy

    Article Title: A serum-free culture medium for long-term expansion of human airway basal stem cells

    doi: 10.1186/s13287-026-04910-z

    Figure Lengend Snippet: Optimization of EGF and FGF10 concentrations for maintaining BSC morphology. A Brightfield images showing the morphology of HBECs cultured in media supplemented with different concentrations of EGF. Scale bar = 200 μm. White boxes demarcate cells exhibiting morphological alterations. B Brightfield images showing the morphology of HBECs cultured in media supplemented with different concentrations of FGF10. Scale bar = 200 μm. White boxes demarcate cells exhibiting morphological alterations. C Immunofluorescence staining of P63 and KRT5 in HBECs grown in medium supplemented with 40 ng/mL FGF10. Scale bar = 50 μm. D Immunofluorescence staining of NGFR in HBECs grown in media supplemented with 40 ng/mL FGF10. White borders demarcate regions with altered NGFR expression patterns. Scale bar = 50 μm

    Article Snippet: The complete sfBSC medium was supplemented with the following components: 1X B27 supplement (Gibco, 17504044), 0.1% recombinant human serum albumin (rHSA, Sigma-Aldrich, A9731), 2 mM Glutamax (Gibco, 35050061), 50 μg/mL L-ascorbic acid (Sigma-Aldrich, A4544), 0.4 μM monothioglycerol (MTG, Sigma-Aldrich, M6145), 1 μg/mL hydrocortisone (STEMCELL Technologies, 07926), 1 ng/mL recombinant human EGF (Sigma-Aldrich, SRP3027), 0.4 ng/mL recombinant human FGF10 (R&D systems, 345-FG), 10 μM Y27632 (Rho-associated protein kinase (ROCK) inhibitor, Selleck, S1049), 1 μM DAPT (γ-secretase/Notch signaling pathway inhibitor, Sigma-Aldrich, D5942), 1 μM A83-01 (ALK5 inhibitor, Sigma-Aldrich, SML0788), and 1 μM DMH-1 (BMP signaling inhibitor, Sigma-Aldrich, D8946).

    Techniques: Cell Culture, Immunofluorescence, Staining, Expressing